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The Complete Mitochondrial Genome of Indigenous Catfish Mystus gulio: Shotgun Assembly, Insight into Structural Characterization and Phylogenetic Relationships

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Abstract

Mystus gulio is a small indigenous euryhaline catfish under the family Bagridae, commonly known as long whiskers catfish. The present study was designed to sequence the whole mitochondrial genome of bagrid catfish (M. gulio) for the first time using the Illumina NGS platform in order to better comprehend the phylogenetic status. The full mitogenome size of M. gulio is 16,554 bp and has been deposited in GenBank with an accession number OQ984891. The M. gulio mitogenomic organization comprised 37 genes in total, including 13 protein-coding genes, 2 ribosomal RNA (rRNA), 22 transfer RNAs (tRNAs), and a D-loop regulatory region, which is comparable to that of typical vertebrate or other fish mitogenomes. All of the genes (28 genes) were encoded on the heavy (H)/positive strand, with the exception of eight tRNA genes and ND6. The entire base composition comprised A 31.88%, T 26.91%, G 15.05%, and C 26.16%, respectively, with a slightly higher A + T content. In phylogenetic analysis, M. gulio is clustered with other Mystus spp., bagrid catfish, and showed the closest genetic relationships. The complete mitogenome information generated in the present study is a source of genetic information for further molecular studies, including conservation strategies.

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Data Availability

The mitochondrial genome sequence data of Mystus gulio, which support this study’s findings, are openly available in the NCBI database (https://www.ncbi.nlm.nih.gov) under the Genbank Accession number OQ984891. The associated BioProject, Bio-Sample, and SRA numbers are PRJNA980917, SAMN35654168, and SRP441460, respectively.

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Acknowledgements

The authors are thankful to the Director of the ICAR-Central Inland Fisheries Research Institute (ICAR-CIFRI) for his support and to the technical assistant (Asim Kumar Jana) for his assistance.

Funding

The financial assistance provided by the Indian Council of Agricultural Research-Central Inland Fisheries Research Institute (ICAR-CIFRI), Barrackpur, India, for carrying out this research work under the Fish Genetic Stock Institute fund (FREM/20-23/17).

Author information

Author notes

  1. Suvra Roy, Pranaya Kumar Parida, and V. L. Ramya have equally contributed to this work.

Authors and Affiliations

  1. Aquatic Environmental Biotechnology (AEB) Division, ICAR- CIFRI, Barrackpore, Kolkata, 700120, India

    Suvra Roy, Pranaya Kumar Parida, Vikash Kumar & Bijay Kumar Behera

  2. Regional Centre of ICAR-CIFRI, Bangalore, 560089, Karnataka, India

    V. L. Ramya

  3. ICAR-Central Inland Fisheries Research Institute (CIFRI), Barrackpore, Kolkata, 700120, India

    Suvra Roy, Pranaya Kumar Parida, Dibakar Bhakta, Bijay Kumar Behera & Basanta Kumar Das

Contributions

SR helped in data analysis and original manuscript preparation; PKP contributed to conceptualization; RK performed in data analysis; DB done sample collection and acquisition; VK was involve in data interpretation, analysis, and editing the manuscript; BKB done supervision; BKD helped in supervision and investigation.

Corresponding authors

Correspondence to Bijay Kumar Behera or Basanta Kumar Das.

Ethics declarations

Conflict of interest

The authors declare no competing interests.

Ethical Approval

This study did not involve humans or animals. This study did not require ethical approval or permission to collect samples.

Additional information

Handling Editor: Wazir S. Lakra .

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 3251 KB)

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Cite this article

Roy, S., Parida, P.K., Ramya, V.L. et al. The Complete Mitochondrial Genome of Indigenous Catfish Mystus gulio: Shotgun Assembly, Insight into Structural Characterization and Phylogenetic Relationships. Agric Res (2025). https://doi.org/10.1007/s40003-025-00856-5

  • Received
  • Accepted
  • Published
  • DOI https://doi.org/10.1007/s40003-025-00856-5

Keywords

  • Mitochondrial genome
  • Mystus spp.
  • Indigenous catfish
  • Phylogeny
  • Genomics
  • Next-generation sequencing

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