The timely volume describes recent discoveries and method developments that have revolutionized Structural Biology with the advent of X-ray Free Electron Lasers. It provides, for the first time, a comprehensive examination of this cutting-edge technology. It discusses of-the-moment topics such as growth and detection of nanocrystals, Sample Delivery Techniques for serial femtosecond crystallography, data collection methods at XFELs, and more. This book aims to provide the readers with an overview of the new methods that have been recently developed as well as a prospective on new methods under development. It highlights the most important and novel Structural Discoveries made recently with XFELS, contextualized with a big-picture discussion of future developments.

Spectroscopy
Femtosecond
Nanocrystals
Biomolecules
Lipidic Cubic Phases
biochemical engineering

• Front Matter
• X-Ray Free Electron Lasers and Their Applications
• Serial Femtosecond Crystallography (SFX): An Overview
• Small Is Beautiful: Growth and Detection of Nanocrystals
• The Lipid Cubic Phase as a Medium for the Growth of Membrane Protein Microcrystals
• Sample Delivery Techniques for Serial Crystallography
• When Diffraction Stops and Destruction Begins
• Climbing the Data Mountain: Processing of SFX Data
• Phasing Serial Crystallography Data
• Structure Determination by Continuous Diffraction from Imperfect Crystals
• Advances in Structure Determination of G Protein-Coupled Receptors by SFX
• Time-Resolved Serial Femtosecond Crystallography, Towards Molecular Movies of Biomolecules in Action
• Towards Molecular Movies of Enzymes
• X-Ray Spectroscopy with XFELs
• Single Molecule Imaging Using X-ray Free Electron Lasers
• The Use of Angular Correlations to Extract Three-Dimensional Structural Information from X-Ray Solution Scattering
• Future Directions of High Repetition Rate X-Ray Free Electron Lasers
• Back Matter

SLAC National Accelerator Laboratory, Menlo Park, USA
Sébastien Boutet, Mark S. Hunter

School of Molecular Sciences, Arizona State University, Tempe, USA
Petra Fromme

Book Title
X-ray Free Electron Lasers

Book Subtitle
A Revolution in Structural Biology

Editors
Sébastien Boutet, Petra Fromme, Mark S. Hunter

DOI
https://doi.org/10.1007/978-3-030-00551-1

Hardcover ISBN
978-3-030-00550-4
Published: 10 January 2019

eBook ISBN
978-3-030-00551-1
Published: 27 December 2018

Edition Number
1

Number of Pages
XVI, 479

Number of Illustrations
20 b/w illustrations, 108 illustrations in colour

Topics
Biochemical Engineering, Spectroscopy/Spectrometry